Isolation of a CEL 1 homolog in tomato (Solanum lycopersicum L.) fruit as a cost-effective endonuclease source for targeting induced local lesions in the genome (TILLING) analysis

Isolation of a CEL 1 homolog in tomato (Solanum lycopersicum L.) fruit as a cost-effective endonuclease source for targeting induced local lesions in the genome (TILLING) analysis

CEL 1, an endonuclease originally purified from celery, has been used in TILLING (Targeting Induced Local Lesions IN the Genome) analysis to cut the hairpin loop and single-strand DNA generated from heteroduplex of mutant and wild DNA molecules. However, one major limitation as with most mutation sc...

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Bibliographic Details
Main Authors: Alcasid, Rochelle E. (Author), Galvez, Hayde F. (Author), Lantican, Darlon V. (Author), Naredo, Maria Elizabeth B. (Author)
Format: Analytics
Language:English
Published: 2019.
Subjects:
Genetics.
Cel 1.
Endo 1.
Single-point mutation.
Tilling.
Tomato.
Online Access:https://forms.gle/KZjBv7aRtY6jiL5E9